{"id":2937,"date":"2017-08-31T12:34:02","date_gmt":"2017-08-31T12:34:02","guid":{"rendered":"http:\/\/health-anything.com\/?p=2937"},"modified":"2022-05-25T14:09:10","modified_gmt":"2022-05-25T14:09:10","slug":"%e7%99%bd%e8%97%9c%e8%8a%a6%e9%86%87%e5%af%b9%e4%ba%ba%e5%ae%ab%e9%a2%88%e7%99%8cc33a%ef%bc%8csiha%e5%92%8chela%e7%bb%86%e8%83%9e%e5%a2%9e%e6%ae%96%e4%b8%8e%e5%87%8b%e4%ba%a1%e7%9a%84%e5%bd%b1","status":"publish","type":"post","link":"http:\/\/johnhealth.blog\/ch\/?p=2937","title":{"rendered":"\u767d\u85dc\u82a6\u9187\u5bf9\u4eba\u5bab\u9888\u764cC33A\uff0cSiHa\u548cHeLa\u7ec6\u80de\u589e\u6b96\u4e0e\u51cb\u4ea1\u7684\u5f71\u54cd"},"content":{"rendered":"

\u4f5c\u8005\uff1a\u7533\u65b0 \u4e8e\u826f \u5f20\u9756 \u674e\u80dc\u7537 \u6f58\u627f\u6069<\/p>\n

\u4f5c\u8005\u5355\u4f4d\uff1a\u897f\u5b89\u4ea4\u901a\u5927\u5b66\u533b\u5b66\u9662\u7b2c\u4e00\u9644\u5c5e\u533b\u9662\uff1a1\u9ebb\u9189\u79d1\uff0c2\u809d\u80c6\u5916\u79d1\uff0c3\u5987\u4ea7\u79d1\uff0c\u9655\u897f \u897f\u5b89 710061<\/p>\n

\u3010\u6458\u8981\u3011\u00a0 \u76ee\u7684\uff1a \u63a2\u8ba8\u767d\u85dc\u82a6\u9187(Res)\u5bf9\u5bab\u9888\u764cC33A\uff0cSiHa\u548cHeLa\u7ec6\u80de\u589e\u6b96\u4e0e\u51cb\u4ea1\u7684\u5f71\u54cd. \u65b9\u6cd5: MTT\u6cd5\u68c0\u6d4b\u4e0d\u540c\u6d53\u5ea6Res\u5bf9C33A\uff0cSiHa\u548cHeLa\u7ec6\u80de\u7684\u6291\u5236\u7387. \u6d41\u5f0f\u7ec6\u80de\u4eea\u68c0\u6d4b\u7ec6\u80de\u7684\u51cb\u4ea1\u7387\u53ca\u7ec6\u80de\u5468\u671f. \u8367\u5149\u663e\u5fae\u955c\u89c2\u6d4b\u51cb\u4ea1\u7ec6\u80de. \u7ed3\u679c: Res\u5728\u4e00\u5b9a\u6d53\u5ea6\u8303\u56f4\u5185\uff0c\u4ee5\u6d53\u5ea6\u548c\u65f6\u95f4\u4f9d\u8d56\u7684\u65b9\u5f0f\u6291\u5236\u5bab\u9888\u764cC33A\uff0cSiHa \u548cHeLa\u7ec6\u80de\u7684\u751f\u957f\uff08P<0.01\uff09. \u8367\u5149\u663e\u5fae\u955c\u4e0b\u7ec6\u80de\u5448\u73b0\u5178\u578b\u7684\u51cb\u4ea1\u6027\u6539\u53d8. Res\u5bf9\u7ec6\u80de\u5468\u671f\u65e0\u660e\u663e\u5f71\u54cd. \u7ed3\u8bba\uff1a Res\u901a\u8fc7\u8bf1\u5bfc\u7ec6\u80de\u51cb\u4ea1\u4ee5\u65f6\u95f4\u4f9d\u8d56\u548c\u6d53\u5ea6\u4f9d\u8d56\u7684\u65b9\u5f0f\u6291\u5236\u5bab\u9888\u764cC33A\uff0cSiHa\u548cHeLa\u7ec6\u80de\u7684\u751f\u957f.<\/p>\n

\u3010\u5173\u952e\u8bcd\u3011\u00a0 \u767d\u85dc\u82a6\u9187\uff1b\u5bab\u9888\u80bf\u7624\uff1b\u7ec6\u80de\u51cb\u4ea1\uff1b\u7ec6\u80de\u589e\u6b96<\/p>\n

\u00a00\u5f15\u8a00<\/h1>\n

\u767d\u85dc\u82a6\u9187\uff08Resveratrol, Res\uff09\u662f\u5e7f\u6cdb\u5b58\u5728\u4e8e\u8461\u8404\u3001\u679c\u7c7b\u3001\u864e\u6756\u7b49\u591a\u79cd\u98df\u7269\u3001\u836f\u7528\u690d\u7269\u4e2d\u7684\u4e00\u79cd\u591a\u915a\u7c7b\u5316\u5408\u7269. \u7814\u7a76\u8868\u660e\uff0cRes\u5177\u6709\u5e7f\u6cdb\u7684\u9884\u9632\u548c\u6297\u80bf\u7624\u4f5c\u7528\uff0c\u5b83\u5bf9\u767d\u8840\u75c5\u3001\u4e73\u817a\u764c\u3001\u5375\u5de2\u764c\u3001\u809d\u764c\u3001\u7ed3\u80a0\u764c\u3001\u9f3b\u54bd\u764c\u3001\u5589\u764c\u3001\u7532\u72b6\u817a\u764c\u7b49\u591a\u79cd\u764c\u7624\u7ec6\u80de\u5177\u6709\u660e\u663e\u7684\u6291\u5236\u4f5c\u7528\uff3b1-6\uff3d. \u4f46\u76ee\u524d\u5bf9Res\u6291\u5236\u5bab\u9888\u764c\u7684\u7814\u7a76\u8fd8\u8fdc\u672a\u6df1\u5165. \u5bab\u9888\u764c(cervical cancer)\u662f\u6700\u5e38\u89c1\u7684\u5973\u6027\u751f\u6b96\u9053\u80bf\u7624\uff0c\u9ad8\u5c45\u53d1\u5c55\u4e2d\u56fd\u5bb6\u5973\u6027\u80bf\u7624\u53d1\u75c5\u7387\u7684\u9996\u4f4d. \u7814\u7a76\u53d1\u73b0\uff0c\u5bab\u9888\u764c\u7684\u53d1\u751f\u4e0e\u4eba\u4e73\u5934\u7624\u75c5\u6bd2\uff08human papilloma virus HPV\uff09\u611f\u67d3\u5bc6\u5207\u76f8\u5173\uff0c\u672c\u5b9e\u9a8c\u9009\u62e93\u79cd\u5bab\u9888\u764c\u7ec6\u80de\u682a\uff0c\u5373\u5bab\u9888\u817a\u764c\u7ec6\u80de\u682aHeLa\u3001\u5bab\u9888\u9cde\u764c\u7ec6\u80de\u682aSiHa\u548cC33A\uff0c\u5176\u4e2dHeLa\u548cSiHa\u4e3a HPV\u9633\u6027\u7684\u7ec6\u80de\u682a\uff0cC33A\u4e3aHPV\u9634\u6027\u7684\u7ec6\u80de\u682a. \u7814\u7a76Res\u5bf9\u4eba\u5bab\u9888\u764c\u7ec6\u80de\u682a\u7684\u589e\u6b96\u4e0e\u51cb\u4ea1\u7684\u5f71\u54cd\uff0c\u65e8\u5728\u63a2\u8ba8Res\u5bf9\u5bab\u9888\u764c\u6f5c\u5728\u7684\u6cbb\u7597\u4f5c\u7528.<\/p>\n

1\u6750\u6599\u548c\u65b9\u6cd5<\/h1>\n

1.1\u6750\u6599\u4eba\u5bab\u9888\u764c\u7ec6\u80de\u682aC33A\uff0cSiHa\u548cHeLa\u7531\u897f\u5b89\u4ea4\u901a\u5927\u5b66\u533b\u5b66\u9662\u5b9e\u9a8c\u533b\u5b66\u4e2d\u5fc3\u60e0\u8d60\uff1bRes\u8d2d\u81eaSigma\u516c\u53f8\uff0c\u7eaf\u5ea699%\uff1bRPMI\ue0111640\u57f9\u517b\u57fa\u8d2d\u81eaInvitrogen\u516c\u53f8\uff1bMTT\u8d2d\u81eaAmressco\u516c\u53f8\uff1bDMSO\u8d2d\u81ea\u5929\u6d25\u5df4\u65af\u592b\u5316\u5de5\u6709\u9650\u516c\u53f8\uff1bAnnexin V\u8367\u5149\u8bd5\u5242\u76d2\u8d2d\u81ea\u6676\u7f8e\u751f\u7269\u6280\u672f\u6709\u9650\u516c\u53f8.<\/p>\n

1.2\u65b9\u6cd5<\/h2>\n

1.2.1\u7ec6\u80de\u57f9\u517b\u4eba\u5bab\u9888\u764cC33A\uff0cSiHa\u548cHeLa\u7ec6\u80de\uff0c\u5728\u542b100 mL\/L\u80ce\u725b\u8840\u6e05\uff0c1\u00d7105 U\/L\u9752\u9709\u7d20\u3001\u94fe\u9709\u7d20\u7684RPMI\ue0111640\u57f9\u517b\u6db2\u4e2d\uff0c37\u2103\uff0c50 mL\/L CO2\u4fdd\u6301\u9971\u548c\u6e7f\u5ea6\u57f9\u517b.<\/p>\n

1.2.2MTT\u6cd5\u68c0\u6d4b\u4e0d\u540c\u6d53\u5ea6Res\u5bf9C33A\uff0cSiHa\u548cHeLa\u7ec6\u80de\u7684\u751f\u957f\u6291\u5236\u4f5c\u7528\u53d6\u5904\u4e8e\u5bf9\u6570\u751f\u957f\u671f\u7684C33A\uff0cSiHa\u548cHeLa\u7ec6\u80de\uff0c\u8c03\u6574\u7ec6\u80de\u5bc6\u5ea6\u4e3a(6~8)\u00d7107\/L\uff0c\u63a5\u79cd\u4e8e96\u5b54\u677f\uff0c\u6bcf\u5b54100 \u03bcL\uff0c\u7ec6\u80de\u8d34\u58c1\u540e\uff0c\u5b9e\u9a8c\u7ec4\u52a0\u5165\u4e0d\u540c\u6d53\u5ea6\u7684Res 100 \u03bcL\uff0c\u4f7f\u5176\u7ec8\u6d53\u5ea6\u5206\u522b\u4e3a12.5\uff0c25\uff0c50\uff0c100\uff0c200\uff0c300\uff0c400\uff0c600 \u03bcmol\/L\uff1b\u540c\u65f6\u8bbe\u7f6e\u7a7a\u767d\u5bf9\u7167\u7ec4\u3001\u6eb6\u5242\u5bf9\u7167\u7ec4\u548c\u8c03\u96f6\u5b54\uff0c\u6bcf\u4e2a\u5242\u91cf\u8bbe3\u4e2a\u5e73\u884c\u5b54. \u57f9\u517b\u677f\u653e\u56de\u5b75\u7bb1\u7ee7\u7eed\u57f9\u517b24\uff0c48\uff0c72 h. \u53d6\u51fa\u57f9\u517b\u677f\uff0c\u6bcf\u5b54\u52a0\u5165\u65b0\u9c9c\u914d\u7f6e\u7684MTT 20 \u03bcL\uff08\u6d53\u5ea6\u4e3a5 g\/L\uff09\uff0c\u7ee7\u7eed\u57f9\u517b4 h\uff0c\u5438\u5f03\u4e0a\u6e05\u6db2\uff0c\u52a0\u5165DMSO 150 \u03bcL\uff0c\u5728\u5e73\u677f\u6447\u5e8a\u644710 min\uff0860\u6b21\/min\uff09\u540e\uff0c\u7528\u9176\u8054\u4eea\u4e8e490 nm\u5904\u6d4b\u51fa\u4e2a\u5b54\u5438\u5149\u5ea6\u503c\uff08A \u503c\uff09. \u7ec6\u80de\u6291\u5236\u7387\uff08%\uff09=\uff081-\u5b9e\u9a8c\u7ec4A\u503c\/\u5bf9\u7167\u7ec4A\u503c\uff09\u00d7100%. \u5b9e\u9a8c\u91cd\u590d3\u6b21.<\/p>\n

1.2.3\u6d41\u5f0f\u7ec6\u80de\u4eea\uff08FCM\uff09Annexin V\u8367\u5149\u67d3\u8272\u68c0\u6d4b\u7ec6\u80de\u51cb\u4ea1\u4ee50\uff0c200\uff0c300 \u03bcmol\/L Res\u5904\u7406\u7ec6\u80de48 h\uff0c\u5206\u522b\u6d88\u5316\u6536\u96c6\u5404\u7ec4\u7ec6\u80de\uff0c\u8c03\u6574\u7ec6\u80de\u5bc6\u5ea6\u81f31\u00d71010 \/L\u5404\u53d6100 \u03bcL\uff1bPBS\u6d17\u6da42\u6b21\u540e\uff0c\u5f03\u4e0a\u6e05\u6db2\uff1b\u52a0\u5165500 \u03bcL\u7ed3\u5408\u7f13\u51b2\u6db2\u60ac\u6d6e\u7ec6\u80de\uff1b\u52a0\u51655\u00a0 \u03bcL Annexin V\ue011EGFP\u6df7\u5300\u540e\uff0c\u52a0\u51655 \u03bcL PI\uff0c\u6df7\u5300\uff1b\u5ba4\u6e29\u3001\u907f\u5149\u67d3\u827215 min\u540e\uff0c\u8fdb\u884c\u8367\u5149\u663e\u5fae\u955c\u548c\u6d41\u5f0f\u7ec6\u80de\u4eea\u7684\u89c2\u5bdf\u548c\u68c0\u6d4b\uff0c\u5206\u6790\u7ec6\u80de\u51cb\u4ea1\u7684\u767e\u5206\u6bd4. \u5b9e\u9a8c\u91cd\u590d3\u6b21.<\/p>\n

1.2.4\u6d41\u5f0f\u7ec6\u80de\u4eeaPI\u67d3\u8272\u68c0\u6d4b\u7ec6\u80de\u5468\u671f\u00a0 \u6d88\u5316\u6536\u96c6\u5404\u7ec4\u7ec6\u80de\uff0c\u57f9\u517b24 h\uff0c\u518d\u5c06\u7ec6\u80de\u9965\u997f24 h\uff08\u5373\u52a0\u5165\u4e0d\u542b\u8840\u6e05\u7684\u57f9\u517b\u6db2\uff09\u540e\uff0c\u4ee50\uff0c200\uff0c300 \u03bcmol\/L Res\u5904\u7406\u7ec6\u80de48 h\uff0c\u5206\u522b\u6d88\u5316\u6536\u96c6\u5404\u7ec4\u7ec6\u80de\uff0c\u8c03\u6574\u7ec6\u80de\u5bc6\u5ea6\u81f31\u00d71010\/L\u5404\u53d6100 \u03bcL\uff1bPBS\u6d17\u6da42\u6b21\u540e\uff0c\u5f03\u4e0a\u6e05\u6db2\uff1b\u52a0\u5165\u9884\u51b7\u7684700 mL\/L\u4e59\u9187\u56fa\u5b9a1 h\uff0c\u79bb\u5fc3\uff0cPBS\u6d17\u6da42 \u6b21\uff0c\u52a0\u5165RNAase\uff0c37\u2103\u6c34\u6d7430 min\u540e\u52a0PI\u67d3\u827215 min\uff0c\u79bb\u5fc3\u540e\u6d41\u5f0f\u7ec6\u80de\u4eea\u68c0\u6d4b\u7ec6\u80de\u5468\u671f. \u5b9e\u9a8c\u91cd\u590d3\u6b21.<\/p>\n

1.2.5\u8367\u5149\u663e\u5fae\u955c\u89c2\u5bdf\u5c06300 \u03bcmol\/L Res\u5904\u7406\u7ec6\u80de48 h\u540e\u67d3\u8272\uff1a\u52a0\u51655 \u03bcL Annexin V\ue011EGFP\uff0c10 \u03bcL PI\u6df7\u5300\uff0c\u5ba4\u6e29\u3001\u907f\u5149\u53cd\u5e9415 min. \u8fdb\u884c\u6fc0\u5149\u5171\u805a\u7126\u663e\u5fae\u955c\u4e0b\u51cb\u4ea1\u7ec6\u80de\u5f62\u6001\u68c0\u6d4b\u4e0e\u89c2\u5bdf.
\n\u7edf\u8ba1\u5b66\u5904\u7406\uff1a\u6240\u6709\u6570\u636e\u7528x\u00b1s\u8868\u793a\uff0c\u91c7\u7528\u65b9\u5dee\u5206\u6790\u8fdb\u884c\u7ec4\u95f4\u6bd4\u8f83\u5206\u6790. P<0.01\u4e3a\u5dee\u5f02\u6709\u7edf\u8ba1\u5b66\u610f\u4e49.<\/p>\n

2\u7ed3\u679c<\/h1>\n

2.1Res\u5bf9\u4eba\u5bab\u9888\u764cC33A\uff0cSiHa\u548cHeLa\u7ec6\u80de\u7684\u751f\u957f\u6291\u5236\u4f5c\u7528Res\u572850 \u03bcmol\/L\u4ee5\u4e0b\u5bf9\u5bab\u9888\u764c\u7ec6\u80de\u7684\u751f\u957f\u65e0\u7edf\u8ba1\u5b66\u5f71\u54cd\uff0cRes\u5728100~600 \u03bcmol\/L\u6d53\u5ea6\u8303\u56f4\u5185\uff0c\u4ee5\u6d53\u5ea6\u548c\u65f6\u95f4\u4f9d\u8d56\u7684\u65b9\u5f0f\u6291\u5236\u5bab\u9888\u764cC33A\u7ec6\u80de\u7684\u751f\u957f\uff08P<0.01\uff09. Res\u5728200~600 \u03bcmol\/L\u6d53\u5ea6\u8303\u56f4\u5185\uff0c\u4ee5\u6d53\u5ea6\u548c\u65f6\u95f4\u4f9d\u8d56\u7684\u65b9\u5f0f\u6291\u5236\u5bab\u9888\u764cSiHa\u7ec6\u80de\u7684\u751f\u957f\uff08P<0.01\uff09. Res\u572850~600 \u03bcmol\/L\u6d53\u5ea6\u8303\u56f4\u5185\uff0c\u4ee5\u6d53\u5ea6\u548c\u65f6\u95f4\u4f9d\u8d56\u7684\u65b9\u5f0f\u6291\u5236\u5bab\u9888\u764cHeLa\u7ec6\u80de\u7684\u751f\u957f\uff08P<0.01\uff0c\u56fe1\uff09.\u00a0 \u88681\u767d\u85dc\u82a6\u9187\u8bf1\u5bfc3\u79cd\u7ec6\u80de\u7684\u51cb\u4ea1\u4f5c\u7528<\/p>\n

2.3\u8367\u5149\u663e\u5fae\u955c\u89c2\u5bdf300 \u03bcmol\/L Res\u5904\u7406\u7ec6\u80de48h\u540e\u7684\u7ec6\u80de\u53ef\u89c1\u5230\u5178\u578b\u7684\u7ec6\u80de\u51cb\u4ea1\u6027\u6539\u53d8\uff0c\u7ec6\u80de\u6838\u7f29\u5c0f\uff0c\u67d3\u8272\u8d28\u51dd\u96c6\uff0c\u7ec6\u80de\u8868\u9762\u5f62\u6210\u7a81\u8d77\uff0c\u7a81\u8d77\u5185\u53ef\u89c1\u88ab\u5305\u88f9\u7684\u7ec6\u80de\u8d28\u4e0e\u7ec6\u80de\u5668\u3001\u6838\u7684\u788e\u7247\u6216\u6574\u4e2a\u6d53\u7f29\u7684\u7ec6\u80de\u6838\uff0c\u90e8\u5206\u7a81\u8d77\u4e0e\u7ec6\u80de\u8131\u79bb\uff0c\u5f62\u6210\u51cb\u4ea1\u5c0f\u4f53\uff08\u56fe3\uff09.<\/p>\n

2.4Res\u5bf9\u4eba\u5bab\u9888\u764cC33A\uff0cSiHa\u548cHeLa\u7ec6\u80de\u5468\u671f\u7684\u5f71\u54cd \u7ecf0\uff0c200\uff0c300 \u03bcmol\/L Res\u5904\u740648 h\u540e\uff0c\u7ec6\u80de\u5468\u671f\u7684\u6539\u53d8\u65e0\u660e\u663e\u5dee\u5f02. C33A\u7684\u7ec6\u80de\u5468\u671fG1\u671f\u5206\u522b(43.66\u00b15.37)%\uff0c(45.08\u00b15.33)%, (49.39\u00b16.27)%. G2\u671f\u5206\u522b\u4e3a(8.95\u00b11.21)%, (3.14\u00b10.87)%, (5.80\u00b10.27)%. S\u671f\u4e3a(47.38\u00b17.55)%, (51.51\u00b17.24)%, (44.81\u00b14.58)%.\u00a0 SiHa\u7684\u7ec6\u80de\u5468\u671fG1\u671f\u5206\u522b\u4e3a(73.75\u00b16.75%), (79.27\u00b18.45%), (79.22\u00b18.90)%. G2\u671f\u5206\u522b\u4e3a(7.53\u00b11.57)%, (3.83\u00b10.83%), (3.71\u00b10.47)%. S\u671f\u4e3a(18.72\u00b12.50)%, (16.90\u00b13.02)%, (17.07\u00b13.17)%. HeLa\u7684\u7ec6\u80de\u5468\u671fG1\u671f\u5206\u522b\u4e3a(67.62\u00b17.63)%, (72.35\u00b18.42)%, (73.64\u00b18.59)%. G2\u671f\u4e3a(11.94\u00b11.98)%, (8.71\u00b11.53)%, (6.56\u00b11.05)%. S\u671f\u4e3a(20.54\u00b13.85)%, (18.85\u00b12.74)%, (19.79\u00b13.34)%.<\/p>\n

3\u8ba8\u8bba<\/h1>\n

Res\u53c8\u79f0\u82aa\u4e09\u915a, \u5b83\u662f\u975e\u9ec4\u916e\u7c7b\u7684\u591a\u915a\u5316\u5408\u7269,\u5316\u5b66\u540d\u79f0\u4e3a(E)\ue0113, 4, 5\ue011\u4e09\u7f9f\u57fa\u4e8c\u82ef\u4e59\u70ef\uff0c\u5206\u5b50\u5f0f\u4e3aC14H12O3\uff0c\u76f8\u5bf9\u5206\u5b50\u8d28\u91cf\u4e3a228.25,\u6709\u6e38\u79bb\u6001\uff08\u987a\u5f0f\ue011\u3001\u53cd\u5f0f\ue011\uff09\u548c\u7cd6\u82f7\u7ed3\u5408\u6001\uff08\u987a\u5f0f\ue011\u3001\u53cd\u5f0f\ue011\uff09\u4e24\u79cd\u6d3b\u6027\u5f62\u5f0f. Res\u662f\u8461\u8404\u5c5e\u690d\u7269\u4ea7\u751f\u7684\u4e00\u79cd\u690d\u7269\u6297\u6bd2\u7d20\uff0c\u5e7f\u6cdb\u5b58\u5728\u4e8e\u81ea\u7136\u754c\u4e2d\uff0c\u662f\u690d\u7269\u5728\u53d7\u5230\u5916\u6765\u4fb5\u5bb3\u65f6\u6240\u4ea7\u751f\u7684\u6297\u4f53\u6027\u7269\u8d28\uff0c\u5177\u6709\u6297\u6c27\u5316\u6d3b\u6027\u3001\u6297\u8840\u5c0f\u677f\u805a\u96c6\u3001\u6297\u52a8\u8109\u7ca5\u6837\u786c\u5316\u3001\u6297\u708e\u3001\u96cc\u6fc0\u7d20\u6837\u6d3b\u6027\u3001\u514d\u75ab\u8c03\u8282\u53ca\u6297\u80bf\u7624\u6d3b\u6027\u7b49. \u8fdb\u4e00\u6b65\u7814\u7a76\u53d1\u73b0\uff0cRes\u901a\u8fc7\u591a\u79cd\u673a\u5236\u53d1\u6325\u5176\u6297\u80bf\u7624\u4f5c\u7528\uff0c\u53ef\u663e\u8457\u6291\u5236\u591a\u79cd\u4eba\u7c7b\u80bf\u7624\u7ec6\u80de\u7684\u751f\u957f\u3001\u8bf1\u5bfc\u80bf\u7624\u7ec6\u80de\u51cb\u4ea1\uff3b1-9\uff3d.<\/p>\n

A:HeLa; B:C33A; C: SiHa.
\n\u56fe3Res\u4f5c\u752848 h\u540e\u8367\u5149\u663e\u5fae\u955c\u4e0b3\u79cd\u7ec6\u80de\u7684\u51cb\u4ea1\u6539\u53d8\u00d7100
\n\u672c\u7814\u7a76\u901a\u8fc7MTT\u6cd5\u68c0\u6d4b\u8868\u660e\uff0c\u5728\u4e00\u5b9a\u7684\u6d53\u5ea6\u8303\u56f4\u5185\uff0cRes\u5bf9\u5bab\u9888\u764c\u7ec6\u80de\u7684\u751f\u957f\u5177\u6709\u660e\u663e\u7684\u6291\u5236\u4f5c\u7528\uff0c\u5728\u5bf9C33A, SiHa, HeLa\u4e09\u79cd\u7ec6\u80de\u7684\u4f5c\u7528\u4e2d\uff0c\u5bf9HPV(\ue011)\u7684C33A\u7ec6\u80de\u7684\u751f\u957f\u6291\u5236\u4f5c\u7528\u6700\u4e3a\u660e\u663e. \u5728HPV(+)\u7684\u4e24\u79cd\u7ec6\u80de\u4e2d\uff0c\u5bf9HeLa\u7ec6\u80de\uff08\u817a\u764c\uff09\u7684\u6291\u5236\u4f5c\u7528\u8f83SiHa(\u9cde\u764c)\u660e\u663e\uff0c\u8fd9\u4e0e\u5316\u7597\u836f\u7269\u5bf9\u5bab\u9888\u817a\u764c\u7684\u6291\u5236\u4f5c\u7528\u5f3a\u4e8e\u5bab\u9888\u9cde\u764c\u6709\u5173. AnnexinV\u662f\u4e00\u79cd\u5206\u5b50\u91cf\u4e3a35~36 ku\u7684Ca2+\u4f9d\u8d56\u6027\u78f7\u8102\u7ed3\u5408\u86cb\u767d\uff0c\u4e0e\u78f7\u8102\u9170\u4e1d\u6c28\u9178\uff08PS\uff09\u6709\u9ad8\u5ea6\u4eb2\u548c\u529b\uff0cPS\u4ece\u7ec6\u80de\u819c\u8102\u8d28\u53cc\u5c42\u7684\u5185\u4fa7\u8fc1\u79fb\u81f3\u5916\u4fa7\u662f\u7ec6\u80de\u51cb\u4ea1\u7ea7\u8054\u53cd\u5e94\u7684\u521d\u59cb\u4e8b\u4ef6\uff0c\u5229\u7528AnnexinV\u9ad8\u5ea6\u4eb2\u548cPS\u53ef\u68c0\u6d4b\u65e9\u671f\u51cb\u4ea1\u7684\u53d1\u751f. \u672c\u7814\u7a76\u4e2d\uff0cAnnexin V\u548cPI\u53cc\u67d3\u63d0\u793aRes\u5904\u7406\u5bab\u9888\u764c\u7ec6\u80de48 h\u540e\uff0c\u51cb\u4ea1\u7ec6\u80de\u6570\u660e\u663e\u589e\u591a,\u8fdb\u4e00\u6b65\u8bc1\u5b9e\u8bf1\u5bfc\u7ec6\u80de\u51cb\u4ea1\u662fRes\u6740\u4f24\u5bab\u9888\u764c\u7ec6\u80de\u7684\u4f5c\u7528\u673a\u5236\u4e4b\u4e00. \u57283\u79cd\u7ec6\u80de\u682a\u4e2d\uff0c\u4f9d\u7136\u662f\u5bf9C33A\u7684\u8bf1\u5bfc\u51cb\u4ea1\u4f5c\u7528\u6700\u4e3a\u663e\u8457\uff0c\u63d0\u793aHPV\u9634\u6027\u7684\u5bab\u9888\u764c\u7ec6\u80de\u5bf9\u5316\u7597\u836f\u7269\u7684\u654f\u611f\u6027\u66f4\u5f3a.
\nRes\u53ef\u660e\u663e\u6291\u5236\u5bab\u9888\u764c\u7ec6\u80de\u7684\u751f\u957f\uff0c\u5176\u6291\u5236\u9014\u5f84\u53ef\u80fd\u4e3a\u901a\u8fc7\u6291\u5236\u7ec6\u80de\u7684\u589e\u6b96\u4f7f\u7ec6\u80de\u751f\u957f\u51cf\u7f13\uff0c\u4e5f\u53ef\u80fd\u662f\u901a\u8fc7\u8bf1\u5bfc\u7ec6\u80de\u51cb\u4ea1\u4f7f\u7ec6\u80de\u6b7b\u4ea1\u52a0\u5feb\uff0c\u4e24\u8005\u7684\u7ed3\u5c40\u662f\u4e00\u81f4\u7684. \u672c\u5b9e\u9a8c\u7814\u7a76\u8868\u660e\uff0cRes\u5bf93\u79cd\u5bab\u9888\u764c\u7ec6\u80de\u682a\u7684\u7ec6\u80de\u5468\u671f\u65e0\u660e\u663e\u6291\u5236\u4f5c\u7528\uff0c\u5b83\u4e3b\u8981\u662f\u901a\u8fc7\u8bf1\u5bfc\u7ec6\u80de\u51cb\u4ea1\u4ece\u800c\u8fbe\u5230\u6291\u5236\u80bf\u7624\u7ec6\u80de\u7684\u4f5c\u7528.<\/p>\n

\u7ec6\u80de\u51cb\u4ea1\u662f\u7ec6\u80de\u81ea\u8eab\u8c03\u8282\u7684\u4e00\u79cd\u7a0b\u5e8f\u6027\u6b7b\u4ea1\u8fc7\u7a0b\uff0c\u5b83\u4e0d\u540c\u4e8e\u7ec6\u80de\u574f\u6b7b\uff0c\u4e0d\u5f15\u8d77\u708e\u75c7\u53cd\u5e94\uff0c\u673a\u4f53\u4ea6\u4e0d\u4f1a\u5f15\u53d1\u4e0d\u826f\u53cd\u5e94. \u56e0\u6b64\uff0c\u8bf1\u5bfc\u7ec6\u80de\u51cb\u4ea1\u662f\u7814\u7a76 \u6cbb\u7597\u80bf\u7624\u7684\u91cd\u8981\u9014\u5f84. Res\u4e0d\u4ec5\u80fd\u6291\u5236\u80bf\u7624\u7ec6\u80de\u7684\u589e\u6b96\u3001\u8bf1\u5bfc\u51cb\u4ea1\uff0c\u800c\u4e14\u80fd\u589e\u5f3a\u653e\u5c04\u8bf1\u5bfc\u7684\u767d\u8840\u75c5\u7ec6\u80de\u682aEOL\ue0111\u51cb\u4ea1\uff3b1\uff3d. \u5728\u67d0\u4e9b\u80bf\u7624\u4e2d\uff0cRes\u662f\u4e00\u79cd\u6f5c\u5728\u7684TRAIL\u654f\u611f\u5242\uff0c\u901a\u8fc7G1\u7ec6\u80de\u5468\u671f\u6355\u83b7\u548cSurvivin\u51cf\u5c11\u589e\u5f3aTRAIL\u8bf1\u5bfc\u7684\u51cb\u4ea1. Survivin\u7684\u4e3b\u8981\u751f\u7269\u5b66\u529f\u80fd\u662f\u6291\u5236\u51cb\u4ea1. \u7ec6\u80de\u51cb\u4ea1\u4e3b\u8981\u6709\u4e24\u6761\u9014\u5f84\uff1a\u6b7b\u4ea1\u53d7\u4f53\u9014\u5f84\u548c\u7ebf\u7c92\u4f53\u9014\u5f84\uff0c\u5747\u6700\u7ec8\u901a\u8fc7\u6fc0\u6d3b\u4e0b\u6e38\u6548\u5e94\u56e0\u5b50\u4f7f\u7ec6\u80de\u51cb\u4ea1. Survivin\u53ef\u4ee5\u76f4\u63a5\u9ecf\u9644\u4e8e\u6d3b\u5316\u7684\u6548\u5e94\u56e0\u5b50\u4e0a\uff0c\u4f7f\u5176\u4e0d\u80fd\u53d1\u6325\u4f5c\u7528\uff0c\u4ece\u800c\u963b\u65ad\u7ec6\u80de\u51cb\u4ea1\u8fc7\u7a0b. Survivin\u5728\u6b63\u5e38\u5bab\u9888\u7ec4\u7ec7\u4e2d\u4e0d\u8868\u8fbe\uff0c\u5bab\u9888\u764c\u7ec4\u7ec7\u4e2dSurvivin\u9ad8\u8868\u8fbe\uff0c\u5728\u6b63\u5e38\u5bab\u9888\u7ec4\u7ec7\u3001\u539f\u4f4d\u764c\u4e0e\u6d78\u6da6\u764c\u4e2d\u5dee\u522b\u6709\u663e\u8457\u6027\u610f\u4e49. Survivin\u57fa\u56e0\u5728\u5bab\u9888\u764c\u7ec4\u7ec7\u4e2d\u7684\u8868\u8fbe\u53ef\u80fd\u6291\u5236\u4e86\u5f02\u5e38\u7a81\u53d8\u7684\u7ec6\u80de\u51cb\u4ea1\uff0c\u6270\u4e71\u4e86\u7ec6\u80de\u751f\u957f\u548c\u51cb\u4ea1\u9014\u5f84\uff0c\u4fc3\u8fdb\u7ec6\u80de\u9003\u79bb\u751f\u957f\u76d1\u63a7\uff0c\u5bfc\u81f4\u80bf\u7624\u7684\u53d1\u751f. Res\u53ef\u901a\u8fc7\u4f7fSurvivin\u51cf\u5c11\u4ee5\u589e\u5f3aTRAIL\u8bf1\u5bfc\u51cb\u4ea1\u7684\u4f5c\u7528\uff0c\u4ece\u800c\u8fbe\u5230\u6291\u5236\u80bf\u7624\u7684\u4f5c\u7528\uff3b10\uff3d. \u5728\u809d\u764c\u7684\u8054\u5408\u5316\u7597\u4e2d\uff0cRes\u4e0e\u7ec6\u80de\u6bd2\u5316\u7597\u836f\u7269\u534f\u540c\u53d1\u6325\u4f5c\u7528\uff0c\u6291\u5236\u751f\u957f\u3001\u8bf1\u5bfc\u51cb\u4ea1\uff3b11\uff3d. \u672c\u5b9e\u9a8c\u8bc1\u660eRes\u80fd\u901a\u8fc7\u8bf1\u5bfc\u7ec6\u80de\u51cb\u4ea1\u4ee5\u6291\u5236\u5bab\u9888\u764cC33A,SiHa,HeLa\u7ec6\u80de\u7684\u751f\u957f\uff0c\u8868\u660eRes\u5728\u5bab\u9888\u764c\u7684\u6cbb\u7597\u4e2d\u6709\u7740\u826f\u597d\u7684\u4e34\u5e8a\u5e94\u7528\u524d\u666f\uff0c\u800cRes\u6291\u5236\u5bab\u9888\u764c\u7ec6\u80de\u751f\u957f\u53ca\u8bf1\u5bfc\u51cb\u4ea1\u7684\u5177\u4f53\u673a\u5236\u8fd8\u6709\u5f85\u4e8e\u8fdb\u4e00\u6b65\u7814\u7a76.<\/p>\n

\u6458\u81ea\uff1a\u5b66\u6c47\u4e50 – \u5b66\u672f\u4ea4\u6d41\u7f51<\/a><\/p>\n

<\/div>\n

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