{"id":18551,"date":"2022-07-31T16:05:15","date_gmt":"2022-07-31T16:05:15","guid":{"rendered":"http:\/\/johnhealth.blog\/ch\/?p=18551"},"modified":"2022-07-31T16:05:15","modified_gmt":"2022-07-31T16:05:15","slug":"%e7%99%bd%e8%97%9c%e8%8a%a6%e9%86%87%e5%a2%9e%e5%bc%ba%e5%a7%9c%e9%bb%84%e7%b4%a0%e5%af%b9raji%e7%bb%86%e8%83%9e%e6%8a%97%e8%82%bf%e7%98%a4%e4%bd%9c%e7%94%a8%e7%9a%84%e6%9c%ba%e5%88%b6%e7%a0%94","status":"publish","type":"post","link":"http:\/\/johnhealth.blog\/ch\/?p=18551","title":{"rendered":"\u767d\u85dc\u82a6\u9187\u589e\u5f3a\u59dc\u9ec4\u7d20\u5bf9Raji\u7ec6\u80de\u6297\u80bf\u7624\u4f5c\u7528\u7684\u673a\u5236\u7814\u7a76"},"content":{"rendered":"<p>\u76ee\u7684<\/p>\n<p>\u7814\u7a76 \u767d\u85dc\u82a6\u9187\u8054\u5408\u59dc\u9ec4\u7d20\u5bf9\u809d\u764c\u51cb\u4ea1\u7684\u5f71\u54cd\u673a\u5236.<\/p>\n<p>\u65b9\u6cd5<\/p>\n<p>\u53d6\u809d\u764c\u7ec6\u80de\u7cfbSMMC-7721,\u6839\u636e\u5904\u7406\u65b9\u5f0f\u7684\u4e0d\u540c\u5206\u4e3a\u5bf9\u7167\u7ec4(A\u7ec4)\u3001 \u767d\u85dc\u82a6\u9187\u7ec4(B\u7ec4)\u3001 \u59dc\u9ec4\u7d20\u7ec4(C\u7ec4)\u4ee5\u53ca\u767d\u85dc\u82a6\u9187+\u59dc\u9ec4\u7d20\u7ec4(D\u7ec4).<\/p>\n<p>\u65b9\u6cd5\u7528\u7ec6\u80de\u8ba1\u6570\u8bd5\u5242\u76d2(cell counting kit-8,CCK-8)\u65b9\u6cd5\u68c0\u6d4b\u5404\u7ec4\u7ec6\u80de\u7684\u589e\u6b96,\u7528\u6bd4\u8272\u6cd5\u68c0\u6d4b\u534a\u80f1\u6c28\u9178\u5929\u51ac\u6c28\u9178\u86cb\u767d\u9176(caspase)-3\u3001caspase-8\u3001caspase-9\u9176\u7684\u6d3b\u6027,\u7528Western\u5370\u8ff9\u65b9\u6cd5\u68c0\u6d4bBax\u3001Bcl-2\u86cb\u767d\u3001 \u805a\u817a\u82f7\u9178\u4e8c\u78f7\u9178\u6838\u7cd6\u8f6c\u79fb\u9176(poly-ADP-ribose polymerase,PARP)\u4ee5\u53caCleaved PARP\u7684\u8868\u8fbe.<!--more--><\/p>\n<p>\u7ed3\u679c<\/p>\n<p>CCK-8\u7ed3\u679c\u663e\u793aD\u7ec4\u7684\u7ec6\u80de\u589e\u6b96\u7387\u6700\u4f4e(P&lt;0.05).\u6d41\u5f0f\u7ec6\u80de\u4eea\u7ed3\u679c\u663e\u793a,D\u7ec4\u7684\u51cb\u4ea1\u7387\u6700\u9ad8.\u6bd4\u8272\u6cd5\u7ed3\u679c\u663e\u793a,D\u7ec4\u7684caspase-3\u3001caspase-8\u3001caspase-9\u9176\u7684\u6d3b\u6027\u6700\u9ad8(P&lt;0.05).Western\u5370\u8ff9\u7ed3\u679c\u663e\u793a,D\u7ec4\u7684Bax\u8868\u8fbe\u9ad8\u4e8eB\u3001C\u7ec4,\u800cBcl-2\u7684\u8868\u8fbe\u4f4e\u4e8eA\u3001B\u3001C\u7ec4(P&lt;0.05);D\u7ec4\u7684PARP\u7684\u8868\u8fbe\u4f4e\u4e8eB\u3001C\u7ec4,\u800cCleaved PARP\u7684\u8868\u8fbe\u9ad8\u4e8eA\u3001B\u3001C\u7ec4(P&lt;0.05).<\/p>\n<p>\u7ed3\u8bba \u767d\u85dc\u82a6\u9187\u548c\u59dc\u9ec4\u7d20\u5747\u80fd\u8bf1\u5bfcSMMC-7721\u7ec6\u80de\u51cb\u4ea1, \u4e24\u8005\u8054\u5408\u5e94\u7528\u540e\u4fc3\u51cb\u4ea1\u4f5c\u7528\u589e\u5f3a.\u5176\u673a\u5236\u53ef\u80fd\u4e0ecaspase\u6d3b\u6027\u5347\u9ad8\u3001Bax\u8868\u8fbe\u589e\u52a0\u3001Bcl-2\u8868\u8fbe\u51cf\u5c11\u4ee5\u53caPARP\u7684\u88c2\u89e3\u6709\u5173\u7cfb.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-large wp-image-18552\" src=\"http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-186-1536x864-1-1024x576.png\" alt=\"\" width=\"900\" height=\"506\" srcset=\"http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-186-1536x864-1-1024x576.png 1024w, http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-186-1536x864-1-300x169.png 300w, http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-186-1536x864-1-768x432.png 768w, http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-186-1536x864-1.png 1536w\" sizes=\"auto, (max-width: 900px) 100vw, 900px\" \/><\/p>\n<p>\u76ee\u7684<\/p>\n<p>\u7814\u7a76\u59dc\u9ec4\u7d20\u5bf9Raji\u7ec6\u80de\u4f53\u5916\u6297\u764c\u4f5c\u7528\u53ca\u5176\u673a\u5236,\u5bf9\u6bd4\u7814\u7a76\u5176\u5bf9Raji\u7ec6\u80de\u548c\u4eba\u5355\u4e2a\u6838\u7ec6\u80de\u7684\u7ec6\u80de\u6bd2\u6027.\u65b9\u6cd5\u7528MTT\u6cd5\u68c0\u6d4b\u59dc\u9ec4\u7d20\u5bf9Raji\u7ec6\u80de\u53ca\u4eba\u5355\u4e2a\u6838\u7ec6\u80de\u589e\u6b96\u7684\u5f71\u54cd,Annexin\u2164\/PI\u53cc\u6807\u6d41\u5f0f\u672f,\u7f3a\u53e3\u672b\u7aef\u6807\u8bb0\u6cd5\u68c0\u6d4b\u59dc\u9ec4\u7d20\u5bf9Raji\u7ec6\u80de\u53ca\u4eba\u5355\u4e2a\u6838\u7ec6\u80de\u51cb\u4ea1\u7684\u5f71\u54cd,PI\u5355\u6807\u6d41\u5f0f\u7ec6\u80de\u672f\u6d4b\u5b9a\u59dc\u9ec4\u7d20\u5bf9Raji\u7ec6\u80deDNA\u542b\u91cf\u5206\u5e03\u7684\u5f71\u54cd.<\/p>\n<p>\u7ed3\u679c<\/p>\n<ul>\n<li>(1)\u59dc\u9ec4\u7d20\u5bf9Raji\u7ec6\u80de\u5177\u6709\u660e\u663e\u7684\u589e\u6b96\u6291\u5236\u4f5c\u7528.<\/li>\n<li>(2)\u59dc\u9ec4\u7d20\u53ef\u4ee5\u65f6\u95f4\u548c\u5242\u91cf\u4f9d\u8d56\u6027\u65b9\u5f0f\u8bf1\u5bfcRaji\u7ec6\u80de\u51cb\u4ea1.<\/li>\n<li>(3)\u59dc\u9ec4\u7d20\u7ec4Raji\u7ec6\u80de\u5468\u671f\u53d1\u751f\u53d8\u5316,\u7ec6\u80de\u5468\u671f\u88ab\u963b\u6ede\u4e8eG0\/G1\u548cG2\/M\u671f,S\u671f\u6bd4\u4f8b\u51cf\u5c11.<\/li>\n<li>(4)\u59dc\u9ec4\u7d20\u5bf9\u4eba\u5355\u4e2a\u6838\u7ec6\u80de\u65e0\u660e\u663e\u6291\u5236\u589e\u6b96\u548c\u8bf1\u5bfc\u51cb\u4ea1\u4f5c\u7528.<\/li>\n<\/ul>\n<p>\u7ed3\u8bba<\/p>\n<p>\u59dc\u9ec4\u7d20\u80fd\u591f\u8c03\u63a7Raji\u7ec6\u80de\u7684\u7ec6\u80de\u5468\u671f\u5e76\u8bf1\u5bfc\u5176\u51cb\u4ea1,\u4ece\u800c\u6291\u5236Raji\u7ec6\u80de\u589e\u6b96;\u59dc\u9ec4\u7d20\u5bf9\u4eba\u5355\u4e2a\u6838\u7ec6\u80de\u65e0\u660e\u663e\u7ec6\u80de\u6bd2\u4f5c\u7528,\u800c\u9009\u62e9\u6027\u4f5c\u7528\u4e8e\u80bf\u7624\u7ec6\u80de.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-large wp-image-18553\" src=\"http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-185-1536x864-1-1024x576.png\" alt=\"\" width=\"900\" height=\"506\" srcset=\"http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-185-1536x864-1-1024x576.png 1024w, http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-185-1536x864-1-300x169.png 300w, http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-185-1536x864-1-768x432.png 768w, http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-185-1536x864-1.png 1536w\" sizes=\"auto, (max-width: 900px) 100vw, 900px\" \/> <img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-large wp-image-18554\" src=\"http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-186-1536x864-2-1024x576.png\" alt=\"\" width=\"900\" height=\"506\" srcset=\"http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-186-1536x864-2-1024x576.png 1024w, http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-186-1536x864-2-300x169.png 300w, http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-186-1536x864-2-768x432.png 768w, http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-186-1536x864-2.png 1536w\" sizes=\"auto, (max-width: 900px) 100vw, 900px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>\u6e90\u81ea\uff1a<a href=\"https:\/\/xueshu.baidu.com\/usercenter\/paper\/show?paperid=df91f4b4447fc85a05d5dcd1c0887423\" target=\"_blank\" rel=\"noopener\">baidu\u00a0\u5b66\u672f<\/a><\/p>\n<div class=\"pvc_clear\"><\/div>\n<p id=\"pvc_stats_18551\" class=\"pvc_stats all  \" data-element-id=\"18551\" style=\"\"><i class=\"pvc-stats-icon medium\" aria-hidden=\"true\"><svg aria-hidden=\"true\" focusable=\"false\" data-prefix=\"far\" data-icon=\"chart-bar\" role=\"img\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\" class=\"svg-inline--fa fa-chart-bar fa-w-16 fa-2x\"><path fill=\"currentColor\" d=\"M396.8 352h22.4c6.4 0 12.8-6.4 12.8-12.8V108.8c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v230.4c0 6.4 6.4 12.8 12.8 12.8zm-192 0h22.4c6.4 0 12.8-6.4 12.8-12.8V140.8c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v198.4c0 6.4 6.4 12.8 12.8 12.8zm96 0h22.4c6.4 0 12.8-6.4 12.8-12.8V204.8c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v134.4c0 6.4 6.4 12.8 12.8 12.8zM496 400H48V80c0-8.84-7.16-16-16-16H16C7.16 64 0 71.16 0 80v336c0 17.67 14.33 32 32 32h464c8.84 0 16-7.16 16-16v-16c0-8.84-7.16-16-16-16zm-387.2-48h22.4c6.4 0 12.8-6.4 12.8-12.8v-70.4c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v70.4c0 6.4 6.4 12.8 12.8 12.8z\" class=\"\"><\/path><\/svg><\/i> <img loading=\"lazy\" decoding=\"async\" width=\"16\" height=\"16\" alt=\"Loading\" src=\"http:\/\/johnhealth.blog\/ch\/wp-content\/plugins\/page-views-count\/ajax-loader-2x.gif\" border=0 \/><\/p>\n<div class=\"pvc_clear\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u76ee\u7684 \u7814\u7a76 \u767d\u85dc\u82a6\u9187\u8054\u5408\u59dc\u9ec4\u7d20\u5bf9\u809d\u764c\u51cb\u4ea1\u7684\u5f71\u54cd\u673a\u5236. \u65b9\u6cd5 \u53d6\u809d\u764c\u7ec6\u80de\u7cfbSMMC-7721,\u6839\u636e\u5904\u7406\u65b9\u5f0f\u7684\u4e0d\u540c\u5206\u4e3a\u5bf9\u7167\u7ec4(A\u7ec4)\u3001 \u767d\u85dc\u82a6\u9187\u7ec4(B\u7ec4)\u3001 \u59dc\u9ec4\u7d20\u7ec4(C\u7ec4)\u4ee5\u53ca\u767d\u85dc\u82a6\u9187+\u59dc\u9ec4\u7d20\u7ec4(D\u7ec4). \u65b9\u6cd5\u7528\u7ec6\u80de\u8ba1\u6570\u8bd5\u5242\u76d2(cell counting kit-8,CCK-8)\u65b9\u6cd5\u68c0\u6d4b\u5404\u7ec4\u7ec6\u80de\u7684\u589e\u6b96,\u7528\u6bd4\u8272\u6cd5\u68c0\u6d4b\u534a\u80f1\u6c28\u9178\u5929\u51ac\u6c28\u9178\u86cb\u767d\u9176(caspase)-3\u3001caspase-8\u3001caspase-9\u9176\u7684\u6d3b\u6027,\u7528Western\u5370\u8ff9\u65b9\u6cd5\u68c0\u6d4bBax\u3001Bcl-2\u86cb\u767d\u3001 \u805a\u817a\u82f7\u9178\u4e8c\u78f7\u9178\u6838\u7cd6\u8f6c\u79fb\u9176(poly-ADP-ribose polymerase,PARP)\u4ee5\u53caCleaved PARP\u7684\u8868\u8fbe.<\/p>\n<div class=\"pvc_clear\"><\/div>\n<p id=\"pvc_stats_18551\" class=\"pvc_stats all  \" data-element-id=\"18551\" style=\"\"><i class=\"pvc-stats-icon medium\" aria-hidden=\"true\"><svg aria-hidden=\"true\" focusable=\"false\" data-prefix=\"far\" data-icon=\"chart-bar\" role=\"img\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\" class=\"svg-inline--fa fa-chart-bar fa-w-16 fa-2x\"><path fill=\"currentColor\" d=\"M396.8 352h22.4c6.4 0 12.8-6.4 12.8-12.8V108.8c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v230.4c0 6.4 6.4 12.8 12.8 12.8zm-192 0h22.4c6.4 0 12.8-6.4 12.8-12.8V140.8c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v198.4c0 6.4 6.4 12.8 12.8 12.8zm96 0h22.4c6.4 0 12.8-6.4 12.8-12.8V204.8c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v134.4c0 6.4 6.4 12.8 12.8 12.8zM496 400H48V80c0-8.84-7.16-16-16-16H16C7.16 64 0 71.16 0 80v336c0 17.67 14.33 32 32 32h464c8.84 0 16-7.16 16-16v-16c0-8.84-7.16-16-16-16zm-387.2-48h22.4c6.4 0 12.8-6.4 12.8-12.8v-70.4c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v70.4c0 6.4 6.4 12.8 12.8 12.8z\" class=\"\"><\/path><\/svg><\/i> <img loading=\"lazy\" decoding=\"async\" width=\"16\" height=\"16\" alt=\"Loading\" src=\"http:\/\/johnhealth.blog\/ch\/wp-content\/plugins\/page-views-count\/ajax-loader-2x.gif\" border=0 \/><\/p>\n<div class=\"pvc_clear\"><\/div>\n","protected":false},"author":1,"featured_media":18552,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[480,77,13,2],"tags":[150,12,5],"class_list":["post-18551","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-480","category-77","category-13","category-2","tag-150","tag-12","tag-5"],"aioseo_notices":[],"_links":{"self":[{"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=\/wp\/v2\/posts\/18551","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18551"}],"version-history":[{"count":1,"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=\/wp\/v2\/posts\/18551\/revisions"}],"predecessor-version":[{"id":18555,"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=\/wp\/v2\/posts\/18551\/revisions\/18555"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=\/wp\/v2\/media\/18552"}],"wp:attachment":[{"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18551"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18551"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18551"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}