{"id":18422,"date":"2022-07-17T21:19:09","date_gmt":"2022-07-17T21:19:09","guid":{"rendered":"http:\/\/johnhealth.blog\/ch\/?p=18422"},"modified":"2022-07-18T12:02:17","modified_gmt":"2022-07-18T12:02:17","slug":"%e3%80%90%e7%a7%91%e6%8a%80%e6%96%87%e7%8c%ae%e3%80%91sirt1%e5%9c%a8%e7%99%bd%e8%97%9c%e8%8a%a6%e9%86%87%e8%b0%83%e6%8e%a7%e9%aa%a8%e8%b4%a8%e7%96%8f%e6%9d%be%e5%a4%a7%e9%bc%a0%e7%9a%84%e6%88%90","status":"publish","type":"post","link":"http:\/\/johnhealth.blog\/ch\/?p=18422","title":{"rendered":"\u3010\u79d1\u6280\u6587\u732e\u3011SIRT1\u5728\u767d\u85dc\u82a6\u9187\u8c03\u63a7\u9aa8\u8d28\u758f\u677e\u5927\u9f20\u7684\u6210\u9aa8\u7ec6\u80de\u7ebf\u7c92\u4f53\u81ea\u566c\u4e2d\u7684\u4f5c\u7528\u53ca\u673a\u5236"},"content":{"rendered":"<div class=\"xx_font\"><span style=\"color: #000;\"><strong>\u3010\u6458\u8981\u3011\uff1a<\/strong><\/span>\u76ee\u7684:\u76ee\u524d\u9aa8\u8d28\u758f\u677e\u7684\u53d1\u75c5\u7387\u9010\u5e74\u4e0a\u5347,\u5df2\u7ecf\u6210\u4e3a\u8001\u5e74\u4eba\u7684\u5e38\u89c1\u75c5\u4e4b\u4e00\u3002\u5176\u539f\u56e0\u5305\u62ec\u793e\u4f1a\u6587\u660e\u548c\u7ecf\u6d4e\u7684\u5feb\u901f\u53d1\u5c55\u3001\u751f\u6d3b\u6c34\u5e73\u7684\u63d0\u9ad8\u3001\u751f\u6d3b\u65b9\u5f0f\u548c\u4e60\u60ef\u7684\u53d8\u5316,\u7279\u522b\u662f\u5728\u53d1\u8fbe\u57ce\u5e02\u548c\u4eba\u53e3\u8001\u9f84\u5316\u65e5\u76ca\u4e25\u91cd\u7684\u5730\u533a\u3002<\/div>\n<div><\/div>\n<div class=\"xx_font\">\u4f17\u6240\u5468\u77e5,\u9aa8\u8d28\u758f\u677e\u75c7\u662f\u4e00\u79cd\u5e74\u9f84\u76f8\u5173\u7684\u5168\u8eab\u6027\u9aa8\u75c5,\u5176\u7279\u70b9\u662f\u9aa8\u91cf\u9010\u6e10\u51cf\u5c11,\u9aa8\u8d28\u8106\u6027\u548c\u6613\u53d1\u751f\u9aa8\u6298\u3002<\/div>\n<div class=\"xx_font\">\u6210\u9aa8\u7ec6\u80de\u662f\u9aa8\u751f\u6210\u7684\u4e3b\u8981\u529f\u80fd\u7ec6\u80de,\u53c2\u4e0e\u9aa8\u7684\u5f62\u6210\u3002\u6210\u9aa8\u7ec6\u80de\u7684\u6d3b\u5316\u4e0d\u8db3\u5bfc\u81f4\u4e0e\u8001\u5316\u76f8\u5173\u7684\u9aa8\u8d28\u758f\u677e\u75c7\u7684\u53d1\u751f\u3002\u589e\u52a0\u6210\u9aa8\u7ec6\u80de\u7684\u6570\u91cf\u53ca\u529f\u80fd\u53ef\u4ee5\u4fc3\u8fdb\u9aa8\u5f62\u6210,\u8fd9\u53ef\u4ee5\u4f5c\u4e3a\u9884\u9632\u548c\u6cbb\u7597\u9aa8\u8d28\u758f\u677e\u75c7\u7684\u4e3b\u8981\u6cbb\u7597\u7b56\u7565\u3002<\/div>\n<p><!--more--><\/p>\n<div><\/div>\n<div class=\"xx_font\">\u76ee\u524d,\u9884\u9632\u548c\u6cbb\u7597\u9aa8\u8d28\u758f\u677e\u75c7\u7684\u836f\u7269\u6cbb\u7597\u5173\u6ce8\u5728\u4fc3\u8fdb\u6210\u9aa8\u7ec6\u80de\u7684\u589e\u6b96\u548c\u5206\u5316\u4e0a\u3002\u81ea\u566c\u662f\u771f\u6838\u7ec6\u80de\u901a\u8fc7\u964d\u89e3\u548c\u56de\u6536\u53d7\u635f\u7684\u5927\u5206\u5b50\u6216\u7ec6\u80de\u5668\u7684\u4e3b\u8981\u964d\u89e3\u4ee3\u8c22\u8fc7\u7a0b\u3002\u5728\u6b63\u5e38\u60c5\u51b5\u4e0b,\u81ea\u566c\u5728\u5927\u90e8\u5206\u7ec6\u80de\u4e0a\u90fd\u662f\u5904\u4e8e\u57fa\u7840\u6c34\u5e73\u7684,\u800c\u5728\u5e94\u6fc0\u7b49\u523a\u6fc0\u4e0b\u7ec6\u80de\u81ea\u566c\u4f1a\u88ab\u6fc0\u6d3b\u4ee5\u8c03\u63a7\u7ec6\u80de\u751f\u5b58\u3002\u6700\u8fd1\u6709\u5927\u91cf\u7814\u7a76\u8868\u660e,\u81ea\u566c\u4f5c\u4e3a\u4e00\u79cd\u7ec6\u80de\u7684\u751f\u5b58\u9014\u5f84,\u5728\u7ef4\u6301\u9aa8\u9abc\u7a33\u6001\u65b9\u9762\u8d77\u7740\u4e3e\u8db3\u8f7b\u91cd\u7684\u4f5c\u7528,\u5e76\u4e14\u8fd9\u4e00\u9014\u5f84\u7684\u6539\u53d8\u4e0e\u9aa8\u8d28\u758f\u677e\u75c7\u7684\u53d1\u751f\u6709\u5173\u3002<\/div>\n<div><\/div>\n<div class=\"xx_font\">\u6c89\u9ed8\u4fe1\u606f\u8f6c\u5f55\u8c03\u8282\u56e0\u5b501(SIRT1)\u5728\u6291\u5236\u80bf\u7624\u3001\u7ef4\u6301\u7ebf\u7c92\u4f53\u7a33\u6001\u3001\u80fd\u91cf\u4ee3\u8c22\u3001\u7ec6\u80de\u8870\u8001\u4ee5\u53ca\u7ec6\u80de\u6b7b\u4ea1\u7b49\u591a\u79cd\u751f\u7269\u5b66\u529f\u80fd\u7684\u8c03\u63a7\u4e2d\u53d1\u6325\u7740\u91cd\u8981\u7684\u4f5c\u7528\u3002\u6700\u8fd1\u7814\u7a76\u53d1\u73b0SIRT1\u5728\u5c0f\u9f20\u4f53\u5185\u7684\u6fc0\u6d3b\u4e0e\u9aa8\u8d28\u758f\u677e\u7b49\u591a\u79cd\u76f8\u5173\u75be\u75c5\u7684\u5ef6\u8fdf\u53d1\u75c5\u6709\u5173\u3002SIRT1\u7684\u7f3a\u5931\u6216\u8fc7\u8868\u8fbe\u5206\u522b\u4f1a\u51cf\u5c11\u6216\u589e\u52a0\u9aa8\u91cf\u3002<\/div>\n<div><\/div>\n<div class=\"xx_font\">\u8fd1\u5e74\u6765,\u767d\u85dc\u82a6\u9187\u5728\u6297\u6c27\u5316\u3001\u6297\u80bf\u7624\u548c\u957f\u5bff\u7b49\u65b9\u9762\u8d77\u7740\u79ef\u6781\u6709\u6548\u7684\u4f5c\u7528\u3002\u6b64\u5916,\u867d\u7136\u767d\u85dc\u82a6\u9187\u662f\u5982\u4f55\u53d1\u6325\u5176\u751f\u7269\u5b66\u529f\u80fd\u5c1a\u4e0d\u5b8c\u5168\u6e05\u695a,\u4f46\u5176\u5bf9SIRT1\u7684\u6fc0\u6d3b\u5df2\u88ab\u8ba4\u4e3a\u662f\u6539\u5584\u7ec6\u80de\u529f\u80fd\u548c\u673a\u4f53\u5065\u5eb7\u7684\u91cd\u8981\u673a\u5236\u3002\u7136\u800c\u767d\u85dc\u82a6\u9187\u6fc0\u6d3bSIRT1\u4f5c\u7528\u4e8e\u6210\u9aa8\u7ec6\u80de\u7684\u673a\u5236\u4ecd\u5f85\u8fdb\u4e00\u6b65\u9610\u660e\u3002<\/div>\n<div><\/div>\n<div class=\"xx_font\">\u672c\u7814\u7a76\u901a\u8fc7\u68c0\u6d4b\u7ec6\u80de\u5185SIRT1,PI3K\/Akt\/m TOR\u4fe1\u53f7\u901a\u8def\u53ca\u81ea\u566c\u76f8\u5173\u86cb\u767d\u5728\u767d\u85dc\u82a6\u9187\u5904\u7406\u7684\u9aa8\u8d28\u758f\u677e\u5927\u9f20\u4e2d\u7684\u8868\u8fbe\u60c5\u51b5,\u5e76\u8fdb\u4e00\u6b65\u901a\u8fc7\u4f53\u5916\u7ec6\u80de\u5b9e\u9a8c,\u89c2\u5bdf\u4e86\u89e3SIRT1\u5728\u767d\u85dc\u82a6\u9187\u5904\u7406\u7684\u7cd6\u76ae\u8d28\u6fc0\u7d20\u578b\u9aa8\u8d28\u758f\u677e\u5927\u9f20\u6210\u9aa8\u7ec6\u80de\u4e2d\u81ea\u566c\u76f8\u5173\u86cb\u767d\u53caPI3K\/Akt\/m TOR\u4fe1\u53f7\u901a\u8def\u7684\u4f5c\u7528,\u4e3a\u4e34\u5e8a\u9884\u9632\u548c\u6cbb\u7597\u9aa8\u8d28\u758f\u677e\u75c7\u63d0\u4f9b\u65b0\u7684\u65b9\u5411\u3002<\/div>\n<div><\/div>\n<div class=\"xx_font\">\u7814\u7a76\u65b9\u6cd5:<\/div>\n<div><\/div>\n<div class=\"xx_font\">\u7b2c\u4e00\u90e8\u5206:\u4f53\u5185\u52a8\u7269\u5b9e\u9a8c<\/div>\n<div><\/div>\n<div class=\"xx_font\">1\u3001\u9aa8\u8d28\u758f\u677e\u52a8\u7269\u6a21\u578b\u7684\u5efa\u7acb\u5c0620\u53ea\u96c4\u6027SD\u5927\u9f20\u968f\u673a\u5206\u4e3a\u4e09\u7ec4,\u5373\u5bf9\u7167\u7ec4(n=5),\u9aa8\u8d28\u758f\u677e\u7ec4(n=5),\u9aa8\u8d28\u758f\u677e+\u767d\u85dc\u82a6\u9187\u7ec4(n=10)\u3002\u5176\u4e2d\u9aa8\u8d28\u758f\u677e\u7684\u5927\u9f20\u6bcf\u5468\u4e24\u6b21\u808c\u6ce8\u5730\u585e\u7c73\u677e5mg\/kg,\u8fde\u7eed\u7ed9\u836f6\u5468,\u8bf1\u5bfc\u9aa8\u8d28\u758f\u677e,\u800c\u5bf9\u7167\u7ec4\u7ed9\u4e88\u6ce8\u5c04\u7b49\u8ba1\u91cf\u7684\u751f\u7406\u76d0\u6c34\u5bf9\u7167\u3002<\/div>\n<div class=\"xx_font\">2\u3001\u5404\u7ec4\u5927\u9f20\u9aa8\u7ec4\u7ec7\u7684\u9aa8\u5bc6\u5ea6,\u80a1\u9aa8\u8fd1\u7aef\u5b54\u9699,\u78b1\u6027\u78f7\u9178\u9176,\u9aa8\u9499\u7d20,SIRT1,\u81ea\u566c\u76f8\u5173\u86cb\u767d\u53caAkt\u548cm TOR\u901a\u8def\u7684\u53d8\u5316\u60c5\u51b5\u9aa8\u8d28\u758f\u677e\u7ec4\u9020\u6a21\u6210\u529f\u540e\u5c06\u9aa8\u8d28\u758f\u677e+\u767d\u85dc\u82a6\u9187\u7ec4\u968f\u673a\u5206\u4e3a\u4f4e\u5242\u91cf\u7ec4(n=5)\u548c\u9ad8\u5242\u91cf\u7ec4(n=5)\u3002\u5728\u9020\u6a21\u6210\u529f\u540e7\u5929,\u5206\u522b\u704c\u80c35mg\/kg\/d\u548c45mg\/kg\/d(\u5373\u4f4e\u5242\u91cf\u7ec4\u548c\u9ad8\u5242\u91cf\u7ec4)\u767d\u85dc\u82a6\u9187\u3002\u9ebb\u9189\u6ee1\u610f\u540e,\u75285ml\u6ce8\u5c04\u5668\u4ece\u53f3\u4fa7\u9888\u603b\u52a8\u8109\u6536\u96c62ml\u8840\u6db2\u6837\u672c\u4e8e\u79bb\u5fc3\u7ba1\u4e2d,\u79bb\u5fc3\u673a\u5206\u79bb,\u7136\u540e\u7528ELISA\u6cd5\u68c0\u6d4b\u5404\u7ec4\u5927\u9f20\u7684\u78b1\u6027\u78f7\u9178\u9176(ALP)\u548c\u9aa8\u9499\u7d20(OC)\u3002\u53d6\u51fa\u80a1\u9aa8,\u7528\u4e8e\u53cc\u80fdX\u7ebf\u5438\u6536\u6cd5\u53ca\u76f8\u5173\u8f6f\u4ef6\u68c0\u6d4b\u5404\u7ec4\u5927\u9f20\u7684\u9aa8\u5bc6\u5ea6\u53ca\u80a1\u9aa8\u8fd1\u7aef\u5b54\u9699\u3002\u91c7\u7528Western blot\u65b9\u6cd5\u6d4b\u5b9a\u5404\u7ec4\u5927\u9f20\u7684\u9aa8\u6807\u672c\u4e2dSIRT1,LC3,Beclin-1,p-m TOR,t-m TOR,p-Akt\u53cat-Akt\u86cb\u767d\u7684\u8868\u8fbe\u60c5\u51b5\u3002<\/div>\n<div><\/div>\n<div class=\"xx_font\">\u7b2c\u4e8c\u90e8\u5206:\u4f53\u5916\u7ec6\u80de\u5b9e\u9a8c<\/div>\n<div><\/div>\n<div class=\"xx_font\">1\u3001\u7ec6\u80de\u7684\u57f9\u517b\u53ca\u5904\u7406\u5c06\u5c0f\u9f20MC3T3-E1\u6210\u9aa8\u7ec6\u80de\u682a\u8fdb\u884c\u4f53\u5916\u57f9\u517b,\u5206\u522b\u7528\u4e0d\u540c\u6d53\u5ea6\u7684\u767d\u85dc\u82a6\u9187(10-8-10-6M)\u5904\u7406\u6210\u9aa8\u7ec6\u80de48h\u548c\u7528\u767d\u85dc\u82a6\u9187(50\u03bcM)\u5904\u7406\u6210\u9aa8\u7ec6\u80de\u540e5min,30min,60min,120min\u540e,\u52a0\u5165\u6216\u4e0d\u52a0\u5165\u767d\u85dc\u82a6\u9187,\u91c7\u7528RT-PCR\u53ca\u514d\u75ab\u8367\u5149\u6280\u672f\u68c0\u6d4b\u6210\u9aa8\u7ec6\u80de\u4e2dSIRT1\u7684\u8868\u8fbe\u60c5\u51b5\u3002\u518d\u5c06\u751f\u957f\u826f\u597d\u7684\u6210\u9aa8\u7ec6\u80de\u682a\u63a5\u79cd\u4e8e96\u5b54\u677f,\u5206\u4e3a\u6b63\u5e38\u5bf9\u7167\u5ea6,\u5730\u585e\u7c73\u677e\u7ec4(DEX),\u767d\u85dc\u82a6\u9187\u7ec4(RES),\u5730\u585e\u7c73\u677e+\u767d\u85dc\u82a6\u9187\u7ec4,\u767d\u85dc\u82a6\u9187+\u5c3c\u514b\u9170\u80fa(\u6216PI3K\u7279\u5f02\u6027\u6291\u5236\u5242\u6216m TOR\u6291\u5236\u5242\u6216p38\u7279\u5f02\u6027\u6291\u5236\u5242\u6216JNK\u7279\u5f02\u6027\u6291\u5236\u5242),\u4f5c\u752848h\u3002<\/div>\n<div><\/div>\n<div class=\"xx_font\">2\u3001\u68c0\u6d4b\u6210\u9aa8\u7ec6\u80deSIRT1,\u81ea\u566c\u76f8\u5173\u86cb\u767d\u53caPI3K\/Akt\/m TOR\u4fe1\u53f7\u901a\u8def\u7684\u53d8\u5316\u60c5\u51b5Brd U\u548cMTT\u6cd5\u68c0\u6d4b\u5404\u7ec4\u6210\u9aa8\u7ec6\u80de\u7684\u589e\u6b96\u548c\u6d3b\u529b\u3002\u7528Western blot\u68c0\u6d4b\u5404\u7ec4\u7684SIRT1,LC3,Beclin-1,Atg7,TOM20,Hsp60,p-m TOR,t-m TOR,p-Akt,t-Akt,p-p38\u548cp-JNK\u86cb\u767d\u8868\u8fbe\u60c5\u51b5\u3002RT-PCR\u68c0\u6d4b\u5404\u7ec4LC3\u548cBeclin-1\u7684m RNA\u7684\u8868\u8fbe\u60c5\u51b5\u3002\u5229\u7528\u900f\u5c04\u7535\u955c\u89c2\u5bdf\u6210\u9aa8\u7ec6\u80de\u7ebf\u7c92\u4f53\u81ea\u566c\u7684\u5f62\u6001\u5b66\u53d8\u5316\u53ca\u6570\u91cf\u7684\u53d8\u5316\u3002<\/div>\n<div><\/div>\n<div class=\"xx_font\">\u7ed3\u679c:<\/div>\n<div class=\"xx_font\">1\u3001\u5728\u9aa8\u8d28\u758f\u677e\u5927\u9f20\u4e2d,\u5176\u9aa8\u5bc6\u5ea6\u4e0e\u5bf9\u7167\u7ec4\u5bf9\u6bd4\u660e\u663e\u964d\u4f4e,\u800c\u4e88\u4ee5\u767d\u85dc\u82a6\u9187\u6cbb\u7597\u7684\u9aa8\u8d28\u758f\u677e\u5927\u9f20\u7684\u9aa8\u5bc6\u5ea6\u5219\u660e\u663e\u7684\u63d0\u9ad8(P0.05)\u3002\u800c\u4e14\u767d\u85dc\u82a6\u9187\u6cbb\u7597\u7ec4\u7684\u80a1\u9aa8\u8fd1\u7aef\u5b54\u9699\u4e5f\u663e\u8457\u964d\u4f4e(P0.05)\u3002\u9aa8\u8d28\u758f\u677e\u5927\u9f20\u4e2d\u8840\u6e05\u7684ALP\u548cOC\u660e\u663e\u9ad8\u4e8e\u5bf9\u7167\u7ec4;\u800c\u4e88\u4ee5\u767d\u85dc\u82a6\u9187\u6cbb\u7597\u540e,\u5176\u9aa8\u8d28\u758f\u677e\u5927\u9f20\u7684ALP\u548cOC\u7684\u8868\u8fbe\u660e\u663e\u964d\u4f4e(P0.05)\u3002<\/div>\n<div class=\"xx_font\">2\u3001\u52a8\u7269\u5b9e\u9a8cWestern blot\u7ed3\u679c\u8868\u660e,\u4e0e\u5bf9\u7167\u7ec4\u76f8\u6bd4,\u9aa8\u8d28\u758f\u677e\u5927\u9f20\u4e2dSIRT1,LC3,Beclin-1\u7684\u8868\u8fbe\u660e\u663e\u964d\u4f4e,p-Akt\u548cp-m TOR\u5219\u660e\u663e\u589e\u9ad8(P0.05),\u800c\u63a5\u53d7\u9ad8\u5242\u91cf\u7684\u767d\u85dc\u82a6\u9187\u6cbb\u7597\u540e\u5176SIRT1,LC3,Beclin-1\u7684\u8868\u8fbe\u660e\u663e\u589e\u52a0,p-Akt\u548cp-m TOR\u5219\u660e\u663e\u964d\u4f4e(P0.05),\u4f46\u5404\u7ec4\u7684t-Akt\u548ct-m TOR\u65e0\u660e\u663e\u8868\u8fbe\u53d8\u5316(P0.05)\u3002<\/div>\n<div class=\"xx_font\">3\u3001\u7ec6\u80de\u5b9e\u9a8c\u90e8\u5206,RT-PCR\u7ed3\u679c\u663e\u793a\u767d\u85dc\u82a6\u9187\u5242\u91cf\u4f9d\u8d56\u6027\u4fc3\u8fdb\u6210\u9aa8\u7ec6\u80de\u7684SIRT1 m RNA\u7684\u8868\u8fbe,\u4e1410-6M\u767d\u85dc\u82a6\u9187\u5177\u6709\u660e\u663e\u7684\u589e\u5f3aSIRT1\u7684\u8868\u8fbe(P0.05)\u3002Western blot\u7ed3\u679c\u663e\u793a\u767d\u85dc\u82a6\u9187\u65f6\u95f4\u4f9d\u8d56\u6027\u4fc3\u8fdb\u6210\u9aa8\u7ec6\u80deSIRT1\u86cb\u767d\u7684\u8868\u8fbe,\u4e14\u572860\u5206\u949f\u540e\u8fbe\u5230\u6700\u9ad8\u5cf0(P0.05),\u4e4b\u540e\u65e0\u660e\u663e\u53d8\u5316\u3002\u514d\u75ab\u8367\u5149\u6cd5\u89c2\u5bdf\u5230,\u4e88\u4ee5\u767d\u85dc\u82a6\u9187\u5904\u7406\u7684\u6210\u9aa8\u7ec6\u80de\u4e2d\u7684SIRT1\u8868\u8fbe\u660e\u663e\u9ad8\u4e8e\u7a7a\u767d\u7ec4(P0.05)\u3002<\/div>\n<div class=\"xx_font\">4\u3001\u4f53\u5916\u57f9\u517b\u6210\u9aa8\u7ec6\u80deWestern blot\u7ed3\u679c\u53d1\u73b0,\u5730\u585e\u7c73\u677e\u53ef\u964d\u4f4e\u6210\u9aa8\u7ec6\u80de\u4e2dSIRT1\u7684\u8868\u8fbe,\u767d\u85dc\u82a6\u9187\u53ef\u4ee5\u4fc3\u8fdb\u6210\u9aa8\u7ec6\u80de\u4e2dSIRT1\u7684\u8868\u8fbe,\u800cNAM\u62b5\u6d88\u767d\u85dc\u82a6\u9187\u5bf9\u6210\u9aa8\u7ec6\u80de\u4e2dSIRT1\u8868\u8fbe\u7684\u5f71\u54cd\u3002Brd U\u548cMTT\u7ed3\u679c\u663e\u793a,\u4e0e\u5bf9\u7167\u7ec4\u76f8\u6bd4,\u7ecf\u5730\u585e\u7c73\u677e\u5904\u7406\u540e\u7684\u7ec6\u80de\u7684\u589e\u6b96\u548c\u6d3b\u529b\u660e\u663e\u964d\u4f4e(P0.05),\u800c\u7ecf\u767d\u85dc\u82a6\u9187\u5904\u7406\u540e\u5219\u663e\u8457\u63d0\u9ad8\u6210\u9aa8\u7ec6\u80de\u589e\u6b96\u548c\u6d3b\u529b(P0.05)\u3002RT-PCR\u7ed3\u679c\u663e\u793a,\u65e0\u8bba\u662f\u5426\u7ecf\u8fc7\u5730\u585e\u7c73\u677e\u5904\u7406,\u7ecf\u8fc7\u767d\u85dc\u82a6\u9187\u5904\u7406\u7ec4\u7684LC3 m RNA\u548cBeclin-1 m RNA\u7684\u8868\u8fbe\u5747\u660e\u663e\u5347\u9ad8(P0.05),\u800cNAM\u5904\u7406\u540e\u5219\u4e0e\u5bf9\u7167\u7ec4\u65e0\u660e\u663e\u53d8\u5316(P0.05)\u3002Western blot\u7ed3\u679c\u663e\u793a\u81ea\u566c\u76f8\u5173\u86cb\u767dLC3\u3001Beclin-1\u548cAtg7\u7684\u8868\u8fbe\u60c5\u51b5\u4e0e\u4e0a\u8ff0\u7ed3\u679c\u76f8\u4e00\u81f4\u3002<\/div>\n<div class=\"xx_font\">5\u3001\u901a\u8fc7\u900f\u5c04\u7535\u955c(TEM)\u7ed3\u679c,\u5730\u585e\u7c73\u677e+\u767d\u85dc\u82a6\u9187\u5904\u7406\u7ec4\u53ef\u89c1\u6bd4\u5730\u585e\u7c73\u677e\u5904\u7406\u7ec4\u7684\u7ebf\u7c92\u4f53\u81ea\u566c\u56ca\u6ce1\u589e\u52a0\u3002Western blot\u7ed3\u679c\u663e\u793a,\u4e0e\u5730\u585e\u7c73\u677e\u5904\u7406\u7ec4\u76f8\u6bd4,\u7ecf\u8fc7\u767d\u85dc\u82a6\u9187\u5904\u7406\u7ec4\u7684TOM20\u548cHsp60\u7684\u86cb\u767d\u6c34\u5e73\u660e\u663e\u964d\u4f4e(P0.05),\u800cNAM\u5904\u7406\u540e\u5219\u4e0e\u5bf9\u7167\u7ec4\u65e0\u660e\u663e\u53d8\u5316(P0.05)\u3002<\/div>\n<div class=\"xx_font\">6\u3001\u901a\u8fc7Western blot\u7ed3\u679c,\u4e0e\u5730\u585e\u7c73\u677e\u5904\u7406\u7ec4\u76f8\u6bd4,\u767d\u85dc\u82a6\u9187\u5904\u7406\u7ec4p-Akt\u548cp-m TOR\u7684\u6d3b\u6027\u5747\u4e0b\u8c03,\u800c\u96f7\u5e15\u9709\u7d20\u548cLY294002\u963b\u65ad\u4e86\u767d\u85dc\u82a6\u9187\u5bf9\u6210\u9aa8\u7ec6\u80de\u4e2dAkt\u548cm TOR\u7684\u78f7\u9178\u5316\u5f71\u54cd,\u4f46\u5404\u7ec4t-Akt\u548ct-m TOR\u7684\u8868\u8fbe\u65e0\u660e\u663e\u5dee\u5f02(P0.05)\u3002\u5bf9\u4e8e\u6709\u65e0\u767d\u85dc\u82a6\u9187\u5e72\u9884\u5904\u7406\u6210\u9aa8\u7ec6\u80de\u540e,p38\u548cJNK\u7684\u86cb\u767d\u6d3b\u6027\u4e0e\u5bf9\u7167\u7ec4\u76f8\u6bd4\u65e0\u660e\u663e\u53d8\u5316\u3002<\/div>\n<div><\/div>\n<div class=\"xx_font\">\u7ed3\u8bba:<\/div>\n<div class=\"xx_font\">1\u3001\u5728\u9aa8\u8d28\u758f\u677e\u5927\u9f20\u4e2d,\u63a5\u53d7\u767d\u85dc\u82a6\u9187\u6cbb\u7597\u540e\u53ef\u660e\u663e\u6539\u5584\u5927\u9f20\u7684\u9aa8\u5bc6\u5ea6\u548c\u80a1\u9aa8\u5b54\u9699\u7387,\u964d\u4f4e\u5927\u9f20\u7684\u9aa8\u8f6c\u5316\u7387,\u51cf\u5c11\u9aa8\u91cf\u6d41\u5931\u3002<\/div>\n<div class=\"xx_font\">2\u3001\u5728\u63a5\u53d7\u767d\u85dc\u82a6\u9187\u6cbb\u7597\u7684\u9aa8\u8d28\u758f\u677e\u5927\u9f20\u4e2d,\u5176SIRT1\u53ca\u76f8\u5173\u81ea\u566c\u86cb\u767d\u8868\u8fbe\u660e\u663e\u589e\u52a0,PI3K\/Akt\/m TOR\u4fe1\u53f7\u901a\u8def\u88ab\u6291\u5236\u3002\u8868\u660e\u767d\u85dc\u82a6\u9187\u5bf9\u9aa8\u8d28\u758f\u677e\u5927\u9f20\u4e2d\u53ef\u80fd\u901a\u8fc7\u589e\u5f3aSIRT1\u7684\u6d3b\u6027,\u8c03\u63a7PI3K\/Akt\/m TOR\u4fe1\u53f7\u901a\u8def,\u4fc3\u8fdb\u7ec6\u80de\u81ea\u566c\u6c34\u5e73\u7684\u589e\u52a0,\u8d77\u5230\u4fdd\u62a4\u9aa8\u8d28\u758f\u677e\u5927\u9f20\u7684\u4f5c\u7528\u3002<\/div>\n<div class=\"xx_font\">3\u3001\u767d\u85dc\u82a6\u9187\u589e\u52a0\u6210\u9aa8\u7ec6\u80deSIRT1\u7684\u6d3b\u6027,\u5728\u66b4\u9732\u4e8e\u5730\u585e\u7c73\u677e\u7ec4\u7684\u6210\u9aa8\u7ec6\u80de\u4e2d,\u767d\u85dc\u82a6\u9187\u589e\u52a0SIRT1\u8868\u8fbe,\u8c03\u63a7PI3K\/Akt\/m TOR\u4fe1\u53f7\u901a\u8def,TOM20\u548cHsp60\u7684\u6d3b\u6027\u964d\u4f4e,\u7ebf\u7c92\u4f53\u81ea\u566c\u6c34\u5e73\u589e\u52a0\u3002\u8868\u660e\u767d\u85dc\u82a6\u9187\u53ef\u589e\u5f3a\u7cd6\u76ae\u8d28\u6fc0\u7d20\u578b\u9aa8\u8d28\u758f\u677e\u5927\u9f20\u7684\u6210\u9aa8\u7ec6\u80de\u5185SIRT1\u86cb\u767d\u7684\u6d3b\u6027\u8868\u8fbe,\u6291\u5236\u7ec6\u80de\u5185PI3K\/Akt\/m TOR\u4fe1\u53f7\u901a\u8def,\u8fdb\u800c\u589e\u5f3a\u7ec6\u80de\u5185\u7ebf\u7c92\u4f53\u81ea\u566c,\u63d0\u9ad8\u6210\u9aa8\u7ec6\u80de\u7684\u589e\u6b96\u548c\u6d3b\u529b\u3002<\/div>\n<div><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-large wp-image-18418\" src=\"http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-1309-1024x576.png\" alt=\"\" width=\"900\" height=\"506\" srcset=\"http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-1309-1024x576.png 1024w, http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-1309-300x169.png 300w, http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-1309-768x432.png 768w, http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-1309-1536x864.png 1536w, http:\/\/johnhealth.blog\/ch\/wp-content\/uploads\/2022\/07\/Screenshot-1309.png 1920w\" sizes=\"auto, (max-width: 900px) 100vw, 900px\" \/><\/div>\n<div class=\"xx_font\"><\/div>\n<div class=\"pvc_clear\"><\/div>\n<p id=\"pvc_stats_18422\" class=\"pvc_stats all  \" data-element-id=\"18422\" style=\"\"><i class=\"pvc-stats-icon medium\" aria-hidden=\"true\"><svg aria-hidden=\"true\" focusable=\"false\" data-prefix=\"far\" data-icon=\"chart-bar\" role=\"img\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\" class=\"svg-inline--fa fa-chart-bar fa-w-16 fa-2x\"><path fill=\"currentColor\" d=\"M396.8 352h22.4c6.4 0 12.8-6.4 12.8-12.8V108.8c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v230.4c0 6.4 6.4 12.8 12.8 12.8zm-192 0h22.4c6.4 0 12.8-6.4 12.8-12.8V140.8c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v198.4c0 6.4 6.4 12.8 12.8 12.8zm96 0h22.4c6.4 0 12.8-6.4 12.8-12.8V204.8c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v134.4c0 6.4 6.4 12.8 12.8 12.8zM496 400H48V80c0-8.84-7.16-16-16-16H16C7.16 64 0 71.16 0 80v336c0 17.67 14.33 32 32 32h464c8.84 0 16-7.16 16-16v-16c0-8.84-7.16-16-16-16zm-387.2-48h22.4c6.4 0 12.8-6.4 12.8-12.8v-70.4c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v70.4c0 6.4 6.4 12.8 12.8 12.8z\" class=\"\"><\/path><\/svg><\/i> <img loading=\"lazy\" decoding=\"async\" width=\"16\" height=\"16\" alt=\"Loading\" src=\"http:\/\/johnhealth.blog\/ch\/wp-content\/plugins\/page-views-count\/ajax-loader-2x.gif\" border=0 \/><\/p>\n<div class=\"pvc_clear\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u3010\u6458\u8981\u3011\uff1a\u76ee\u7684:\u76ee\u524d\u9aa8\u8d28\u758f\u677e\u7684\u53d1\u75c5\u7387\u9010\u5e74\u4e0a\u5347,\u5df2\u7ecf\u6210\u4e3a\u8001\u5e74\u4eba\u7684\u5e38\u89c1\u75c5\u4e4b\u4e00\u3002\u5176\u539f\u56e0\u5305\u62ec\u793e\u4f1a\u6587\u660e\u548c\u7ecf\u6d4e\u7684\u5feb\u901f\u53d1\u5c55\u3001\u751f\u6d3b\u6c34\u5e73\u7684\u63d0\u9ad8\u3001\u751f\u6d3b\u65b9\u5f0f\u548c\u4e60\u60ef\u7684\u53d8\u5316,\u7279\u522b\u662f\u5728\u53d1\u8fbe\u57ce\u5e02\u548c\u4eba\u53e3\u8001\u9f84\u5316\u65e5\u76ca\u4e25\u91cd\u7684\u5730\u533a\u3002 \u4f17\u6240\u5468\u77e5,\u9aa8\u8d28\u758f\u677e\u75c7\u662f\u4e00\u79cd\u5e74\u9f84\u76f8\u5173\u7684\u5168\u8eab\u6027\u9aa8\u75c5,\u5176\u7279\u70b9\u662f\u9aa8\u91cf\u9010\u6e10\u51cf\u5c11,\u9aa8\u8d28\u8106\u6027\u548c\u6613\u53d1\u751f\u9aa8\u6298\u3002 \u6210\u9aa8\u7ec6\u80de\u662f\u9aa8\u751f\u6210\u7684\u4e3b\u8981\u529f\u80fd\u7ec6\u80de,\u53c2\u4e0e\u9aa8\u7684\u5f62\u6210\u3002\u6210\u9aa8\u7ec6\u80de\u7684\u6d3b\u5316\u4e0d\u8db3\u5bfc\u81f4\u4e0e\u8001\u5316\u76f8\u5173\u7684\u9aa8\u8d28\u758f\u677e\u75c7\u7684\u53d1\u751f\u3002\u589e\u52a0\u6210\u9aa8\u7ec6\u80de\u7684\u6570\u91cf\u53ca\u529f\u80fd\u53ef\u4ee5\u4fc3\u8fdb\u9aa8\u5f62\u6210,\u8fd9\u53ef\u4ee5\u4f5c\u4e3a\u9884\u9632\u548c\u6cbb\u7597\u9aa8\u8d28\u758f\u677e\u75c7\u7684\u4e3b\u8981\u6cbb\u7597\u7b56\u7565\u3002<\/p>\n<div class=\"pvc_clear\"><\/div>\n<p id=\"pvc_stats_18422\" class=\"pvc_stats all  \" data-element-id=\"18422\" style=\"\"><i class=\"pvc-stats-icon medium\" aria-hidden=\"true\"><svg aria-hidden=\"true\" focusable=\"false\" data-prefix=\"far\" data-icon=\"chart-bar\" role=\"img\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\" class=\"svg-inline--fa fa-chart-bar fa-w-16 fa-2x\"><path fill=\"currentColor\" d=\"M396.8 352h22.4c6.4 0 12.8-6.4 12.8-12.8V108.8c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v230.4c0 6.4 6.4 12.8 12.8 12.8zm-192 0h22.4c6.4 0 12.8-6.4 12.8-12.8V140.8c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v198.4c0 6.4 6.4 12.8 12.8 12.8zm96 0h22.4c6.4 0 12.8-6.4 12.8-12.8V204.8c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v134.4c0 6.4 6.4 12.8 12.8 12.8zM496 400H48V80c0-8.84-7.16-16-16-16H16C7.16 64 0 71.16 0 80v336c0 17.67 14.33 32 32 32h464c8.84 0 16-7.16 16-16v-16c0-8.84-7.16-16-16-16zm-387.2-48h22.4c6.4 0 12.8-6.4 12.8-12.8v-70.4c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v70.4c0 6.4 6.4 12.8 12.8 12.8z\" class=\"\"><\/path><\/svg><\/i> <img loading=\"lazy\" decoding=\"async\" width=\"16\" height=\"16\" alt=\"Loading\" src=\"http:\/\/johnhealth.blog\/ch\/wp-content\/plugins\/page-views-count\/ajax-loader-2x.gif\" border=0 \/><\/p>\n<div class=\"pvc_clear\"><\/div>\n","protected":false},"author":1,"featured_media":18418,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[2],"tags":[5,304,87],"class_list":["post-18422","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-2","tag-5","tag-304","tag-87"],"aioseo_notices":[],"_links":{"self":[{"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=\/wp\/v2\/posts\/18422","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18422"}],"version-history":[{"count":2,"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=\/wp\/v2\/posts\/18422\/revisions"}],"predecessor-version":[{"id":18452,"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=\/wp\/v2\/posts\/18422\/revisions\/18452"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=\/wp\/v2\/media\/18418"}],"wp:attachment":[{"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18422"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18422"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/johnhealth.blog\/ch\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18422"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}